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Image Search Results
Journal: Analytical chemistry
Article Title: Liquid chromatography - high resolution mass spectrometry analysis of fatty acid metabolism
doi: 10.1021/ac202220b
Figure Lengend Snippet: Peak intensities of (A) long-chain fatty acids (C14–C24) and (B) very-long-chain fatty acids (C26–C36) from immortalized baby mouse kidney epithelial cells with or without expression of activated Ras. The cells were grown to 80% confluence in 6 cm culture dishes in DMEM containing 10% dialyzed serum and unlabeled glucose and glutamine. The extracted and saponified fatty acids were resuspended in 1:1:0.3 chloroform/methanol/water solution to a final concentration of 2 μl packed cell volume per 1 ml solvent for analysis of the more abundant long-chain fatty acids, and to a 45-fold higher concentration (90 μl cell volume per 1 mL solvent) for analysis of the less abundant very-long-chain fatty acids, and samples analyzed by LC-MS. Resulting peak heights were corrected for residual differences in total fatty acids injected between the two cell lines by correcting individual peak intensities with the total ion current (TIC) for a given sample. Bars indicate mean ± SD (N=3). * indicate significant differences in FA levels between parental and activated Ras expressing cells (p < 0.05, unpaired two-tailed t-test equal variance, Bonferroni corrected).
Article Snippet:
Techniques: Expressing, Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Injection, Two Tailed Test
Journal: Analytical chemistry
Article Title: Liquid chromatography - high resolution mass spectrometry analysis of fatty acid metabolism
doi: 10.1021/ac202220b
Figure Lengend Snippet: 13C-labeling patterns for very-long-chain monounsaturated fatty acids from immortalized baby mouse kidney epithelial cells with and without expression of activated Ras. Cells were maintained for 72 hour in DMEM with U-13C-glucose and U-13C-glutamine and 10% dialyzed FBS. In the schematic drawing of the fatty acids, * represent 13C atoms. The double bonds are shown at their most likely position but have not been experimentally determined. Bars indicate mean ± standard deviation (N=2).
Article Snippet:
Techniques: Labeling, Expressing, Standard Deviation